BIOTIN ASSAY MEDIUM (Milieu test pour biotine)
Milieu destiné à la détermination microbiologique de la concentration en biotine.

------------------------------------------------------------------------------------------

Biotin Assay Medium

Intended Use
Biotin Assay Medium is used for determining biotin concentration by the microbiological assay technique.

Summary and Explanation
Vitamin assay media are used in the microbiological assay of vitamins. Three types of media are used for this purpose:
1. Maintenance Media: For carrying the stock culture to preserve the viability and sensitivity of the test organism for its intended purpose;
2. Inoculum Media: To condition the test culture for immediate use;
3. Assay Media: To permit quantitation of the vitamin under test. They contain all the factors necessary for optimal growth of the test organism except the single essential vitamin to be determined.

Biotin Assay Medium is prepared for use in the microbiological assay of biotin using Lactobacillus plantarum ATCC™ 8014 as the test organism.

Principles of the Procedure
Biotin Assay Medium is a biotin-free dehydrated medium containing all other nutrients and vitamins essential for the cultivation of L. plantarum ATCC™ 8014. The addition of a biotin standard in specified increasing concentrations gives a growth response by this organism that can be measured titrimetrically or turbidimetrically.

Formula [ Difco™ Biotin Assay Medium ]
Approximate Formula* Per Liter
 - Vitamin Assay Casamino Acids : 12.0 g
 - Dextrose : 40.0 g
 - Sodium Acetate : 20.0 g
 - L-Cystine : 0.2 g
 - DL-Tryptophan : 0.2 g
 - Adenine Sulfate : 20.0 mg
 - Guanine Hydrochloride : 20.0 mg
 - Uracil : 20.0 mg
 - Thiamine Hydrochloride : 2.0 mg
 - Riboflavin : 2.0 mg
 - Niacin : 2.0 mg
 - Calcium Pantothenate : 2.0 mg
 - Pyridoxine Hydrochloride : 4.0 mg
 - p-Aminobenzoic Acid : 200.0 μg
 - Dipotassium Phosphate : 1.0 g
 - Monopotassium Phosphate : 1.0 g
 - Magnesium Sulfate : 0.4 g
 - Sodium Chloride : 20.0 mg
 - Ferrous Sulfate : 20.0 mg
 - Manganese Sulfate : 20.0 mg
*Adjusted and/or supplemented as required to meet performance criteria.