DEV Tryptophan Broth
This culture medium is intended for growing intermediate cultures in the differentiation of coliform bacteria when carrying out the bacteriological analysis of water as recommended in the German Standard Methods and the German drinking water regulation (1990).

The broth complies with the German regulations for the examination of foods.

Typical Composition (g/litre)
Peptone from meat 10.0; DL-tryptophan 1.0; sodium chloride 5.0.

Preparation
Suspend 16 g/litre, dispense into suitable containers, autoclave (15 min at +121°C).
pH: 7.2 ± 0.2 at +25°C.
The prepared broth is clear and yellowish-brown.

Experimental Procedure and Evaluation
The broth is inoculated with the pure culture to be tested and incubated according to DEV 4 to 6 hours at +35°C aerobically.

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25°C): 7.0 - 7.4

Quality control : Test strains / Growth / Indole formation
 - Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good / Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
 - Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good / Indole formation (Escherichia coli ATCC 11775 (WDCM 00090)): +
 - Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good / Indole formation (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): -
 - Growth (Proteus mirabilis ATCC 14153): good to very good / Indole formation (Proteus mirabilis ATCC 14153): -
 - Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good / Indole formation (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Incubation: 4 -6 hours ; +35°C ; aerobic.

Storage temperature : +15°C to +25°C

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Poids	0,8 Kg   (800 g)
Volume	0,0056 m³   (5600 cm³)
Largeur	0,15 m   (15 cm)
Longueur	0,15 m   (15 cm)
Hauteur	0,25 m   (25 cm)

Les dimensions, le poids et le volume sont bruts (emballage inclus)