Alkyl chains of various length, such as C18, C8 and C4 for example, are commonly used for bonding during the synthesis of reversed phase materials of different polarity. YMC however, applies another approach for creating divergent polarities and improving the consistency in the synthesis of reversed phase packings. YMC keeps the C18-alkyl chain length constant, but specifically changes content of C18 groups at the silica surface, yielding three different J´sphere ODS packings with graduated hydrophobicity

Features:
    high quality reversed phase columns
    polarity range created solely by C18 bonding density
    additional selectivity through H-bonding

The exclusive use of C18 groups makes the hydrophobic interaction identical for all three types of J´sphere ODS packings; only the degree of hydrohobicity, i.e. the polarity, is varied.

In addition to the hydrophobic interaction, the surface siloxane groups of J’sphere ODS packings provide a pronounced H-bonding capacity contributing additional selectivity. The ability to interact strongly via H-bonding creates the opportunity to make use of an additional degree of freedom in selectivity. The “controlled hydrogen bonding capacity" of YMC J’sphere ODS packings represents an efficient tool for the chromatographic discrimination of closely related compounds presenting only minor molecular differences.

The recommended pH range of using YMC J’sphere ODS-M80 and -L80 columns is pH 2-7.5 and pH 1-9 for J’sphere ODS-H80. Remove acid and buffer salts before storage. Store the column in methanol/water = 50/50. Clogged inlet frits often can be cleaned by changing the flow direction or replacement.

Technical informations :
% Carbon Load : 9
Chemistry : C18
Endcapped : Yes
Format : Column
Inner Diameter (id) : 4.6 mm
Length (mm) : 150 mm
Mode : Reversed-Phase
Particle Shape : Spherical
Particle Size : 4 µm
Particle Substrate : Silica
Pore Size : 80 Å
Surface Area : 510 m²/g
USP Classification : L1
pH Range : 2 - 7.5